University · Chemical Engineering · Separation Processes

Chromatographic Separations and Preparative Scale-Up

4 Abschnitte

Plate theory and height equivalent to a theoretical plate (HETP), van Deemter equation, gradient elution and isocratic mode, ion-exchange, hydrophobic-interaction, and size-exclusion chromatography, scale-up from analytical to preparative scale, and pharmaceutical bioseparation by protein A affinity chromatography.

Inhaltsübersicht

  • Plate Theory and the van Deemter Equation
  • Chromatography Modes: Ion-Exchange, HIC, and Size-Exclusion
  • Gradient Elution and Isocratic Mode
  • Scale-Up from Analytical to Preparative Chromatography and Protein A Affinity
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