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Metabolic Engineering and Pathway Design

4 Abschnitte

Metabolic flux analysis (MFA) and flux balance analysis (FBA), genetic engineering approaches for pathway insertion and deletion, cofactor balance and redox constraints, directed evolution and rational protein design, production of bio-based chemicals (1,3-propanediol, succinic acid, lactic acid) in engineered microorganisms.

Inhaltsübersicht

  • Metabolic Flux Analysis and Flux Balance Analysis
  • Genetic Engineering Strategies: Pathway Insertion, Deletion, and Optimisation
  • Cofactor Balance, Redox Constraints, and Thermodynamic Feasibility
  • Bio-Based Chemicals: Case Studies in 1,3-Propanediol, Succinic Acid, and Lactic Acid
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